FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32965224
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT448672513.610479334222028No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2218890.6731002343560596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1109810.3366608399202748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG908840.2756965946902105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG907560.2753083067174062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCTCGAGTCATACCCGA828220.25124051940311404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT797390.2418882395581477No Hit
GCGCGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740940.22476413325752012No Hit
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC535050.16230740613198927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG521080.15806960692880473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCCTGAAGCTGACAGCA475050.1441064074067872No Hit
CGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425270.1290056454644446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGGCTCTTGGTGGTACC409130.12410957680736524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACTCGGTGGTGGCCACTG369140.11197861115701808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGCGCGCGCGCGCGCG341560.10361221874300021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9418850.067.8863141
GAGTACA4523250.066.31299620
AGTACAT4525500.066.1942721
GTACATG4512250.066.09032422
TACATGG4519250.065.92374423
ACATGGG4512250.065.9128824
ATCAACG9669300.065.81103511
TCAACGC9664700.065.78918512
CAACGCA9683250.065.76113
GTGGTAT9680150.065.633566
AGCAGTG9728800.065.616112
TGGTATC9686100.065.541067
GGTATCA9708150.065.515278
AGTGGTA9710900.065.495515
GTATCAA9722500.065.454179
AGAGTAC9685350.065.4200419
CATGGGG2898600.065.3968425
AACGCAG9730200.065.3966214
TATCAAC9747750.065.3204910
CGCAGAG9720050.065.3174416
Produced by FastQC (version 0.11.5)