FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005777552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005777552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32811164
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22535256.868165359814727No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1750240.5334281953544836No Hit
CGCGCGCGCGCGCGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG777180.23686450136301168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT634090.19325434477118825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT445600.13580743432326875TruSeq Adapter, Index 7 (97% over 36bp)
ACTGCTCGAGTCATACCCATACGATGTTCCAGATTACGCTATATGTGAAC376810.11484200926245712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA353880.10785353424218659No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC347890.10602793610126114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4978050.066.6591641
GAGTACA2553900.065.34930420
GTACATG2556850.064.9686622
TACATGG2559100.064.7870923
AGTACAT2570500.064.7734421
ACATGGG2563950.064.4720624
ATCAACG5145200.064.43911
TCAACGC5147950.064.4062212
CAACGCA5147350.064.3948713
GTGGTAT5169100.064.152436
GGTATCA5176250.064.064728
TGGTATC5189750.063.8912357
GTATCAA5197350.063.838129
AGTGGTA5202200.063.7577865
AGCAGTG5205750.063.7222562
AACGCAG5202450.063.6950614
AGAGTAC5202450.063.56789419
CGCAGAG5211400.063.5344316
ACGCAGA5221000.063.4371515
TATCAAC5230150.063.43280810
Produced by FastQC (version 0.11.5)