FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361383
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG10400.28778332129624246No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG5600.15496024992874596No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA4430.12258462628291869No Hit
CCCAAGAGGATCTTCATTGGCCCCACCCCCTGCCCTTCCTGTGGCTCATCACCGTCTGAGGAACTGGAGCTCCAT4420.12230791155090306No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGACTTCCGATCAATAGGTTTGATGGC4080.11289961066237204No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC3980.11013246334221588No Hit
TGTACACATCAGCTCAGCCCGAAGTGGGGTGAAGATCTCCCAATTGACAC3810.10542831289795038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGA252.4817473E-588.4496770
CGGTCCG306.129117E-573.7080570
CTACCCG307.430572E-873.2977968
GCGCTAT353.2341632E-971.2116267
TATCACG150.002169088270.4397665
ACCGACC150.002169088270.4397665
CACGATA150.00220565270.1435462
TTGATCG150.002227809569.96760
TAGAACG207.939682E-569.96699560
TTCGCGT150.002233993769.9181259
TTACTCG150.002245157569.8303157
TACCGGC150.002245157569.8303157
CGGTTAT208.128423E-569.63595654
CCGGATA150.002281413269.5488552
GTGTCGC301.0752956E-769.49090650
GCGTACG208.212819E-569.490950
ATTGTCG301.0804797E-769.4426949
CGCGTAC208.241105E-569.4426949
GTTACGC150.002306670269.35606447
GCAACCG150.002316831169.2792544
Produced by FastQC (version 0.11.5)