FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863356
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT5100.065.582639
GCGCATT8000.052.4835479
CGCAATT9300.050.503579
CCGTATT7900.049.5446439
ACGTATT8200.048.5998889
GCGGATT7950.048.33799
CGTGATT5700.047.442759
GCGAATT8350.046.022319
CGCGAAT4950.043.1297729
TCGCATT7150.042.7980049
CGCTATT7600.042.1366549
CGCGCTA3900.041.964197
CGTCATT12050.041.3401579
TCGCGAA3800.041.1959658
CGGCATT11250.041.1170549
ACGCGAT3300.040.968998
ACGGATT9250.040.005789
CGGTATT9300.039.790699
CGATATT9450.039.1590969
ACGCATT8400.038.970839
Produced by FastQC (version 0.11.5)