FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4811520
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG115680.24042298483639268No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA76290.15855696328810853No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA71360.14831072093641925No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGACTTCCGATCAATAGGTTTGATGGC65050.13519636206437882No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC62740.13039538441074755No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG61760.1283586060122373No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC50430.10481095371109338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA1200.089.4489170
CGCGTAT2100.076.0275569
CGCATAA900.069.4588532
CGACGGT1550.067.216148
ACGTACG1100.066.2988712
GTACGCG900.065.6000219
GCGTAAC900.065.59730514
GTGCGAA1750.065.4918126
CGCGCAA800.065.1203841
TAGAACG2650.064.33234460
CGCGATA1100.063.3729665
CGGCTAG2150.062.99952325
TGCGAAC2150.062.99428616
TGCGCGA1550.062.88210762
CGCGTAC1400.062.04455649
TACGCGC900.061.74248520
CGCGATC2500.061.12124312
GCGCGAA1600.060.94688463
TCGCGTA401.1223165E-860.86695559
GACGCGT800.060.84542557
Produced by FastQC (version 0.11.5)