FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10237831
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT854600.065.6572117
GGAGTCC756600.065.0833816
CGAGTCC206200.064.701616
AGTCCTC584850.064.3175118
TCGAGTC153050.063.56495715
GAGTCCA377100.062.59041217
ATTGGAG505950.062.3762213
TGGAGTC547750.062.31603215
GAGTCCG151950.062.0141917
TTCGAGT128800.061.9262214
ATTCGAG155150.061.87267713
TTAGTCC172000.061.5358316
GAGTACT185250.061.0775817
AGTCCTA221700.060.964318
AGAGTCC275100.060.63047816
GTCCTCT294000.060.5194319
TTGGAGT450650.060.34974714
AGTCCGA106600.059.8557118
TTTAGTC185700.059.72857715
TAGTCCT192400.059.34997617
Produced by FastQC (version 0.11.5)