FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4786564
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT13600.053.8084539
CGCTATT14950.053.7018749
CGTTATT13100.052.065759
CGCAATT15250.051.713669
CGCGATT9400.051.3964589
CGGTATT18100.046.711159
ACGTATT13350.046.300989
GCGTATT13050.046.276519
GCGGATT14750.043.8330359
CGCGTAT5200.043.7209638
GCGAATT13650.043.2013789
CGGCATT20150.042.3114939
ACGCATT12350.041.9960149
CGCGAAT6850.041.4879159
CCCGATT12350.041.4207279
CGTAATT18050.041.3298959
CGATATT16500.040.0452659
CGTGATT10650.038.6928379
CGGGATT18200.038.6470159
ACGGATT15750.037.8922969
Produced by FastQC (version 0.11.5)