FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19112133
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT1555800.065.435817
CGAGTCC370900.064.990716
GGAGTCC1370350.064.6623516
TCGAGTC276200.064.1322915
AGTCCTC1064750.063.9380418
GAGTCCA680250.062.39678617
TTAGTCC306300.062.00891516
GAGTCCG283050.061.99283617
ATTGGAG918250.061.8138213
TGGAGTC984350.061.71416515
TTCGAGT236650.061.69745314
AGAGTCC516700.061.55200616
GAGTACT334550.061.24337817
ATTCGAG289350.061.04641313
AGTCCTA401750.060.7460918
GTCCTCT563050.060.19157819
AGTCCGA196900.059.97673418
TAGAGTC302200.059.97240415
TTTAGTC330650.059.76551415
TTGGAGT825700.059.65582314
Produced by FastQC (version 0.11.5)