FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6277787
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT11450.059.4648939
GCGCATT17650.049.8278249
CGCTATT17700.049.6870659
CGCAATT18500.049.455319
GCGTATT20350.048.4445959
ACGGATT16950.047.2828529
CGATATT20950.046.041549
CGGTATT20600.044.758049
GCGAATT19350.043.9840479
CGTTATT19550.043.896879
GCGGATT18650.043.733479
CGTAATT22000.042.876959
TCGCATT15050.041.9419339
CCGTATT16600.040.1619389
CGGAATT27400.040.1214039
CGTGATT14500.040.1089029
CGACATT16150.039.9635289
CGCGTAT5950.039.3345878
ACGTATT18350.038.2643249
CGGGATT21200.038.1385279
Produced by FastQC (version 0.11.5)