FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096857
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT4850.058.3394179
CGCTATT9500.058.0784269
GCGGATT11200.056.8418669
CGGTATT11300.053.8348929
GCGCATT10100.052.5271389
CGGGATT11400.052.1216629
ACGGATT9900.050.0157369
CGATATT9350.049.931689
CGTTATT9700.049.5885059
GCGTATT10900.047.3740129
GCGAATT9950.046.9207239
CGCTAAT6450.044.9643149
CGTAATT11100.044.608639
CCGTATT9700.044.4838079
CGCAATT9150.043.292319
AGCGATT8750.042.037729
CGTGATT6900.042.0318579
CGACATT9450.041.9179539
CGGAATT16150.040.7337389
ACGTATT9550.039.9976279
Produced by FastQC (version 0.11.5)