FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11617074
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG286050.24623239896724422No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA198080.17050765106600851No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA172780.14872936162754924No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC151930.13078164088478733No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG150250.12933549360191732No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGACTTCCGATCAATAGGTTTGATGGC136690.11766301910446642No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC123640.10642955360360105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA2100.079.29835570
CGCGTAT3750.069.5154569
ATTCGCG2800.066.972726
GTCGCGT2450.066.6238224
TCGCGAC2700.066.2875469
CGACGGT3800.064.882518
TACGCGA2100.064.4966920
CGCGTAC2700.064.3428649
CGAATCG2650.064.216726
GCGTACG2750.063.1784350
CGGTACG1550.062.76028448
CGCGAAT3000.062.7125164
CGTACGG2400.062.22330530
CGCGAAA4700.061.32542414
TACGGTA3700.061.1193260
ACGTACG2850.060.9230612
CGCGCTA2000.060.7880339
GTACGCG2400.060.7744219
CGTACCG2350.060.59230823
AGTACGC3400.060.45813464
Produced by FastQC (version 0.11.5)