FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15181623
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG398870.2627321202746241No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA245680.16182723019798345No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA197850.13032203473897355No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC191820.12635012738756587No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGACTTCCGATCAATAGGTTTGATGGC177950.11721408178822514No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG167630.11041638960472146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA3250.091.3775170
CGCGTAT6200.072.0780469
TCGCGAC3150.065.3825969
CGCGATA3750.065.0244565
TACGCGC3700.064.7710420
CGTACGG2400.063.6760830
TACGGTA5550.063.29512460
GTACGCG3900.063.2301719
TACGCGA2750.063.1496220
ACGTACG3900.062.3373412
TAGCGTA1850.062.0417660
CGACGGT6450.061.9227338
CGATTAG3500.061.53570646
TGCGAAC7800.061.4478216
ATTCGCG4200.061.192586
GCGTACG4150.061.116350
CGCGAAT5250.060.3593164
TTCGCGT4850.060.229159
TACGTTA3300.059.99036814
TCGTACG2550.059.9304329
Produced by FastQC (version 0.11.5)