FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15181623
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC315100.064.4762816
TCGAGTC233650.064.1720815
GAGTCCA590600.062.7676117
TTCGAGT194450.062.4276914
ATTCGAG238950.062.29380413
GAGTACT260650.062.28862417
TTAGTCC254800.061.95697816
AGAGTCC433000.061.62901316
TAGAGTC248550.061.36013415
AGTCCTC872850.060.27489518
GAGTCCG231100.060.2493217
TTTAGTC276800.059.83262615
TTGAGTC280100.059.53728515
AGTACTC201800.059.4703718
GAGTCCT1398900.059.30205517
TAGTCCT285800.059.1185317
TGAGTCC341650.058.83487316
TATTCGA105050.058.80086512
ATCGAGT93850.058.6350914
AGTCCGA150350.058.50164418
Produced by FastQC (version 0.11.5)