FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278205
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT107650.064.611217
AGTCCTC69550.064.37147518
TCGAGTC18750.063.95515415
CGAGTCC26100.063.7900216
GGAGTCC93750.063.58440416
GAGTCCA50550.063.1930217
ATTCGAG20200.062.46158613
TTCGAGT16200.062.22153514
AGTCCTA26350.062.00003418
TTAGTCC21050.061.59060316
AGAGTCC37700.061.4953416
ATTGGAG64150.061.22651313
TTTAGTC22250.061.08076515
GAGTACT23050.060.92354217
TGAGTCC28100.060.73414616
TTGAGTC23600.060.67963815
TAGAGTC20800.060.6598115
TAGTCCT22650.060.3113517
GTCCTCT33050.059.94847519
TGGAGTC66250.059.6005415
Produced by FastQC (version 0.11.5)