FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45596273
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC1177850.064.1786216
TCGAGTC738450.062.21706415
AAACGAG1051600.061.716113
AACGAGT753750.061.47098514
GAGTCCG744950.060.3294917
AAAGGAG2259350.059.4197413
AAGGAGT1650350.059.30737714
ACGAGTC637400.059.06038715
ATTCGAG632000.058.81102813
TTCGAGT564800.058.1350414
AGTCCGA429950.057.8589118
AGAGTCC929600.057.66275816
AGGAGTC1474750.057.03880315
GAGTCCA1071600.056.9808317
TAAACGA428400.056.8828512
AATCGAG332600.055.39472213
AATGGAG1397950.054.94486613
GAGTACT557350.054.8182317
AAACGAC368500.054.77416213
AATGGCG335350.054.73302513
Produced by FastQC (version 0.11.5)