FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7216611
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC177550.065.3015616
TCGAGTC139800.064.9557115
TTCGAGT108450.063.24611714
GAGTCCA294600.062.61868317
TTAGTCC108050.062.323716
ATTCGAG117300.062.12274613
AGAGTCC205550.062.10616
GAGTACT139700.061.94381317
GAGTCCG112000.061.5565317
TAGAGTC129200.061.48583215
GAGTCCT613700.060.5720517
AGTCCTC384150.060.3597418
AGTCCGA76100.059.94275318
TTTAGTC127850.059.68774815
TAGTCCT121800.059.6865117
ATCGAGT51200.059.54783214
GGAGTCC552650.059.1450516
AGTACTC99950.058.99708618
AATCGAG66000.058.9445413
AGTCCTA162950.058.65538818
Produced by FastQC (version 0.11.5)