FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32639470
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC896900.065.9190216
TCGAGTC671800.065.2824615
GAGTCCT2897450.065.0306217
GGAGTCC2452300.063.84817516
GAGTCCA1410100.063.47353717
AGTCCTC1892100.063.40823718
TTCGAGT524100.062.80862414
AGAGTCC1028950.062.55788816
GAGTACT707200.062.52348317
ATTCGAG578000.062.41571413
AGTCCTA763700.062.30237218
TAGAGTC609950.061.95047815
GAGTCCG524450.061.1214217
AGTCCGA359050.061.0053918
TGGAGTC1771650.060.95183615
TTAGTCC526400.060.8137916
AATCGAG316700.060.40896613
ATTGGAG1563950.060.39719413
AGTACTC487250.060.3886218
CGAGTAC216950.060.14112516
Produced by FastQC (version 0.11.5)