FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11345465
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG170610.1503772652773597No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC118910.10480839701149314No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA114760.10115054781800481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA4150.063.6682370
CGCGTAT5300.061.4317269
CGTACGG2900.056.28404230
ACGTACG3700.055.37829212
TCGTACG3050.052.3773429
TAGAACG7050.051.87114760
CGGCTAG4800.051.3691725
CGCGATC4600.051.33816512
CGCGATA2450.051.28702565
ACGGAGT16800.049.81857717
GTTAGCG4050.049.73435616
CGCGAAT1700.049.25242664
CGAATCG2550.049.02840426
TAGACGC24800.048.66733655
CGCATCG2750.047.99771545
TACGCGA1600.047.7514120
TAACCGC16750.047.47928622
TCGTAAG3700.046.9336541
GTGCGAA4900.046.777126
CGGGATT5150.046.52938528
Produced by FastQC (version 0.11.5)