FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21977019
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG316600.1440595742306998No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA264500.12035299236898325No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA262360.11937924793166899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA13100.084.4438370
CGCGTAT15000.069.2005369
TAGCGTA12300.056.68148860
TGCGAAC17500.054.94972216
ACGGAGT46400.054.91637817
CGATTAG13550.052.8044146
TAACCGC33100.050.65763522
CGCGATC10700.050.2890812
CGTACGG5050.050.18307530
TTCGCGT6100.049.69688459
CCGTCTA14000.049.59372327
TCGCGAC4550.049.55711469
AGACGCG39050.049.42885211
TCGTACG5350.049.31562829
TACGTTC22200.048.78961612
CGATCTA15000.048.46272370
CGACGGT5950.048.4275868
CGCGATA4800.048.06472865
CGGATTA33250.047.8197731
TAGAACG12050.047.732460
Produced by FastQC (version 0.11.5)