FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21977019
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC663800.065.6481616
GAGTCCT1868400.064.3536217
TCGAGTC406750.063.84161415
GGAGTCC1595950.063.1811216
GAGTCCG385750.062.37607617
AAACGAG555300.062.23394413
AACGAGT411450.062.17403414
AGTCCTC1009550.061.483218
ATTCGAG355300.061.43716413
TTCGAGT311500.061.23261614
ACGAGTC354500.061.10486615
AAGGAGT874500.060.37475614
AAAGGAG1191750.060.2376413
AGTCCTA467600.059.69170418
AGTCCGA222000.059.4882318
AGAGTCC494000.059.303916
GAGTCCA577200.058.89649617
AGGAGTC794550.058.8199315
TGGAGTC999900.058.78943315
GTCCTCT528450.057.28124619
Produced by FastQC (version 0.11.5)