FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14676402
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTC239100.064.498715
CGAGTCC321500.064.3830216
TTCGAGT203850.062.8762514
GAGTCCA581000.062.7289117
TTAGTCC247150.062.34226616
ATTCGAG245400.062.24532713
AGAGTCC435750.061.99875616
TAGAGTC248550.061.5568815
AGTCCTC864650.061.52005818
GAGTACT267600.061.11382717
GAGTCCT1382900.061.04525817
TTTAGTC265850.060.95108815
GGAGTCC1223750.059.72636816
TAGTCCT280950.059.36068717
GAGTCCG228150.059.2771617
AGTCCTA347800.059.21764418
ATCGAGT96050.059.06728714
AGTACTC203000.059.03518318
AGTCCGA148000.058.3754618
AATCGAG134600.058.1885313
Produced by FastQC (version 0.11.5)