FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241294
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT7800.055.2998549
GCGTATT13500.053.9500969
GCGGATT14700.053.3942459
CGGTATT15450.053.0906759
CGTTATT12400.051.3227129
CGATATT14750.049.8574569
ACGTATT11500.048.575589
CGCAATT13350.048.2002079
TCGCATT9100.048.1769269
CGTGATT10550.047.5876969
CGCGTAT5650.047.5563558
ACGGATT13150.047.3201039
CGCTATT12600.046.0184569
CGGGATT18250.045.7201779
CCGTATT11600.043.8897679
ACGCATT11300.043.8034559
CGTAATT15700.043.6878669
GCGAATT12750.043.258679
GCGCATT11800.040.748889
GGCGTAT7100.039.8359388
Produced by FastQC (version 0.11.5)