FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22722429
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG352280.15503624194402807No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA301670.13276309500185918No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA293730.1292687502731332No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC251830.11082882028149367No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG241060.10608901011419157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA8900.066.3313970
CGCGTAT12150.064.6540469
ACGGAGT46950.055.6226417
GGGTACG10700.053.69570
TAGAACG13450.052.0663460
TGCGAAC15100.051.74566716
TAGACGC59100.051.67555655
CGCGATA4450.051.00095465
ACGTACG6850.050.18995712
GCGTACG5750.050.16621850
CGCGATC11250.049.69876512
TAACCGC38750.049.1109322
TAGCGTA5450.048.58479360
TTAGACG62900.048.3769954
CGGATCG8950.048.11361317
AGACGCG42200.047.64761711
GCGTTAT18900.047.6416664
CGGTACG3850.046.9264348
TCGCACG9600.046.3026914
CGGCTAG8950.046.1735525
Produced by FastQC (version 0.11.5)