FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8835920
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC257050.065.19424416
TCGAGTC160800.063.1922915
GAGTCCT762550.062.4818517
AAACGAG221050.062.47147413
AACGAGT163800.062.05615214
ATTCGAG140950.061.77910613
GAGTCCG159850.061.34884317
GGAGTCC676750.061.07181516
TTCGAGT125450.060.84624514
ACGAGTC136550.060.7897915
AGTCCTC406900.060.7647718
AAGGAGT356550.060.01176514
AAAGGAG488300.059.90801613
AGTCCTA180900.059.592718
AGAGTCC193350.059.2087116
GAGTCCA241200.059.02560817
TAAACGA86050.058.59890412
AGGAGTC327650.058.39562615
AGTCCGA89150.057.3800918
GAGTACT115300.057.15442317
Produced by FastQC (version 0.11.5)