FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005784998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005784998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18012700
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG265880.14760696619607278No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA228350.12677166665741393No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA223140.12387926296446397No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC190580.10580312779316814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT8200.061.54384669
GCGTATA5600.060.6524570
ACGGAGT40500.056.5780217
ACGTACG5950.053.68301812
CGCGATC8600.052.07836512
TAGACGC40250.050.9093755
CGGATCG7250.050.76059717
TGCGAAC10850.050.5574316
CGCGAAT3200.050.1856964
CGAATCG4000.049.47362526
TAGAACG12700.048.8424460
GCGTACG4200.048.81598350
AGACGCG35400.048.54776411
GCGTTAT12350.047.7740264
TAACCGC31050.047.63287722
GTACGCG3750.047.216819
TCGCACG6400.047.19536214
TACGGTA5300.046.68351760
TTAGACG44450.046.32030554
GACGCGT5500.046.2016557
Produced by FastQC (version 0.11.5)