FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5063885
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC137550.065.1596716
GAGTCCT386550.064.6016817
GGAGTCC331650.063.6446916
TCGAGTC86650.063.6188415
GAGTCCG82150.062.1503417
AAACGAG123700.061.9966513
AGTCCTC215250.061.57732818
AACGAGT88000.061.26019314
ATTCGAG74250.061.2221313
TTCGAGT65100.060.6913314
AGTCCTA91750.060.19625518
AAGGAGT189050.059.57026314
AAAGGAG261300.059.47070713
ACGAGTC72700.059.32152615
TGGAGTC211500.059.0842715
AGAGTCC107900.058.82561516
AGGAGTC165500.058.6105215
AGTCCGA48600.057.6798118
GAGTCCA126600.057.4166217
GAGTACT61650.056.69672817
Produced by FastQC (version 0.11.5)