FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4700225
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC121900.064.05086516
TCGAGTC76550.062.4243415
AAACGAG109250.061.94892513
GAGTCCG76200.061.7069517
AACGAGT78150.060.96828514
AAAGGAG236850.059.79250713
AAGGAGT173350.059.64629414
ACGAGTC66000.059.28174215
ATTCGAG64500.059.0427813
AGAGTCC97200.058.71576316
TAAACGA45400.058.3729312
GAGTCCA118100.057.98400517
TTCGAGT57250.057.89349714
AGTCCGA43150.057.78912718
AATCGAG37050.057.72970213
AGGAGTC159100.057.35959215
AACGACT30550.056.57929614
AAACGAC38600.056.4026413
ATCGAGT26350.056.35853214
AATGGAG147950.056.27578713
Produced by FastQC (version 0.11.5)