FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655055
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT6800.070.353139
CGTTATT11050.057.301199
CGGTATT14500.056.7676969
CGATATT12450.056.5085339
ACGTATT10900.056.153419
CGCGTAT4550.052.5686878
CCGTATT12800.052.215219
GCGTATT12400.051.0627569
GCGATAT7450.050.0471348
GCGCATT11250.050.0288939
CGCAATT11250.050.0288939
AGCGATT9050.049.752499
CGCGTTA5450.049.05027
CGCTAAT7000.047.23719
CGCTATT10250.046.6732949
CGTAATT13600.046.0399139
ACGCGAT3850.045.6813478
ACGGATT12000.044.5569769
CGTGATT9100.044.067349
CCGGATT16350.043.8900229
Produced by FastQC (version 0.11.5)