FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7461608
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG179930.24114105163391053No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGACTTCCGATCAATAGGTTTGATGGC109810.14716666970443903No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA109410.14663059222623326No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA93020.12466481755675184No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG85210.11419790479478419No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC80020.10724229951506431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA1150.088.891570
CGCGTAT2350.072.7805769
TCGCGAC1050.070.3387969
ATCGCGA1150.066.7789866
CGTACGG1200.066.569630
GCGTAAC1000.065.9898514
CGCGAAA2750.065.6741114
ATTCGCG2150.064.622826
CGCGAAT2500.064.09022564
TCGTACG1250.063.90853529
ACGTACG1850.063.8308712
CGTAAGT2350.063.55126616
CGACGGT2850.063.368918
TAGAACG3900.063.32845760
TCGCGAA2050.062.68738635
TGCGAAC4000.062.51670516
TACGTTA1900.062.15111514
CGCGATT850.061.37891459
TTCGCGT2050.061.07950259
TCGCGTA800.060.86742459
Produced by FastQC (version 0.11.5)