FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7461608
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC201750.065.5787716
TCGAGTC150100.064.9621215
GAGTCCT647100.064.7290617
GGAGTCC549500.063.6348716
AGTCCTC437150.063.43710718
ATTCGAG130200.063.27226313
TTCGAGT117650.063.2533114
GAGTCCA311600.063.24536517
GAGTACT158900.062.48186517
TAGAGTC128850.062.4560615
AGAGTCC223650.061.9860916
AGTCCTA166600.061.80794518
TGGAGTC403500.061.16779715
GAGTCCG117250.061.1357817
AGTACTC117950.061.06973618
TTAGTCC122000.060.7484816
AATCGAG69600.060.4299313
AGTCCGA78200.060.2374818
ATTGGAG347350.060.1527413
TTGAGTC141350.059.66271615
Produced by FastQC (version 0.11.5)