FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14020029
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC251300.064.4632116
TCGAGTC190000.064.3973715
GAGTCCT1099050.064.0369917
GGAGTCC991800.063.59878516
AGTCCTC757600.063.2632718
GAGTCCA488650.062.31230517
TTAGTCC233100.061.32208616
TTCGAGT163900.061.28540414
GAGTCCG202450.061.26348517
ATTCGAG200750.061.20633713
GAGTACT237950.060.83269517
AGAGTCC355900.060.5724916
TGGAGTC708000.060.4199215
ATTGGAG658450.060.2540513
TTTAGTC251700.060.11849215
AGTCCTA280400.060.03070418
GTCCTCT392650.059.36758819
AGTCCGA141500.059.2211618
TAGTCCT262700.058.79424317
TTGAGTC249500.058.4885515
Produced by FastQC (version 0.11.5)