FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7195524
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG103860.1443397311995624No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA101090.1404901157997666No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA85580.11893504906661419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA2850.064.07265570
CGCGTAT4000.062.76493569
CGCGAAA3050.057.92754414
TCGTACG1350.056.45516229
CGGATCG2200.055.11377717
ACGTACG2150.054.78457312
ACGGAGT14250.054.21316517
AGACGCG15450.052.9177411
TCGCACG2500.052.6572714
TGCGAAC4050.048.75673716
ACTAGCG1800.048.57539459
TAACCGC12850.048.52707722
CGTACGG1650.048.29049730
TAGACGC17150.047.9458655
CGCGATC3700.047.75141512
CGACGGT2400.047.6343778
CGCTATA13100.047.6009926
GACGCGC16900.047.55750712
TCGTAAG2950.046.9901441
GTCGCGT2900.046.58878324
Produced by FastQC (version 0.11.5)