FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4267087
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8850.052.1611379
CGATATT14300.051.6504679
GCGCATT13750.049.5844549
CGACATT11300.047.765269
ACGGATT13100.047.7076879
CGCAATT13550.047.6956839
CGCGTAT4800.047.3434948
ACGTATT13000.046.9820639
GCGTATT12700.046.9734579
CGTTATT13050.046.2578479
CCGTATT12400.045.8189669
CGGTATT14750.045.259829
CGCTATT12550.044.705449
CCCGATT12050.044.202949
GCGAATT14350.044.046879
TCGCATT10050.042.399649
CGTAATT14650.042.1753439
CGTGATT9300.040.473429
ACGCATT12400.040.0915959
CGGAATT21350.037.9213649
Produced by FastQC (version 0.11.5)