FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610231
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG78330.16990471844035582No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA61680.13378939146433227No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC60920.13214088404680807No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA59610.12929937784028608No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG50700.10997279745852213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA1050.080.9813470
CGTACGG1000.065.9623530
CGCGTAT2000.063.95923269
TCGCGTA601.8189894E-1258.05314359
GCGTACG1500.057.91322750
CGCGTAC1450.057.50570749
CGCGATC2700.056.5771312
ACGGAGT10250.055.8871617
TACGGTA1200.055.17751360
TGCGAAC2850.053.59938416
CGCGAAT1250.053.09933564
ACGTACG1050.052.90328612
AGACGCG8850.052.5670311
CGATAAG1550.051.51726532
TAACCGC9400.050.9675522
CGCATCG1300.050.7545945
CCGATCG1100.050.5770454
TAGAACG3350.049.93282760
TAGACGC12650.049.7637755
GTTAGCG1200.049.18352516
Produced by FastQC (version 0.11.5)