FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610231
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC126150.064.31924416
TCGAGTC79400.062.9871315
AAACGAG110100.062.2605213
AACGAGT78950.061.98054514
GAGTCCG78050.060.5563917
AAGGAGT172100.060.2413214
ACGAGTC65450.059.88632615
AAAGGAG239150.059.81384713
ATTCGAG64900.059.48283813
TAAACGA42700.059.21339812
AGAGTCC97500.058.92761216
TTCGAGT57900.058.7453814
GAGTCCA117550.058.4625517
GAGTCCT393800.057.97039817
AGGAGTC157250.057.83549515
AGTCCTC205350.057.68443318
AGTCCGA42800.056.96170418
AACGACT29150.056.91061414
GAGTACT55150.056.6927617
AATCGAG37500.056.20238513
Produced by FastQC (version 0.11.5)