FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5070489
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC98500.065.06232516
TCGAGTC73650.064.0689415
GAGTCCT417700.064.0519617
GGAGTCC376100.063.4898316
AGTCCTC283800.063.44518718
GAGTCCA183300.062.60315717
ATTCGAG74300.062.3833913
TTCGAGT63000.062.36852614
GAGTCCG76150.061.5095317
AGAGTCC133600.061.2689516
AGTCCTA102700.061.11612318
TAGAGTC76150.060.73264315
TTAGTCC84350.060.6000116
GAGTACT87400.060.59449417
AGTCCGA51950.060.57759518
TGGAGTC270050.060.0944115
ATTGGAG249700.060.0325913
TTTAGTC92150.059.2440515
GTCCTCT151400.058.6826619
TAGTCCT95050.058.4248417
Produced by FastQC (version 0.11.5)