FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14129113
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC385050.065.52814516
GAGTCCT1250150.065.4643417
TCGAGTC291300.065.3773815
GGAGTCC1060750.064.36040516
GAGTCCA599100.063.79440317
AGTCCTC843900.063.7649518
TTCGAGT222200.063.72567414
AGTCCTA316050.062.8692818
ATTCGAG245600.062.72484613
GAGTACT303200.062.7226217
AGAGTCC425100.062.4207916
TAGAGTC256300.062.23520315
TTAGTCC230850.061.98964716
TGGAGTC772050.061.54907615
ATCGAGT107250.061.3142314
ATTGGAG662050.061.00229313
AGTACTC215450.060.55278818
TTTAGTC256600.060.46919315
TAGTCCT271950.060.35643417
TTGAGTC277900.060.32472615
Produced by FastQC (version 0.11.5)