FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4320195
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC111350.065.09231616
GAGTCCT309400.064.56785617
TCGAGTC68100.063.2260115
GGAGTCC270800.062.97025316
AACGAGT72450.061.78206314
AAACGAG100800.061.4850313
GAGTCCG69450.060.3444117
AGTCCTC169950.060.30887218
ACGAGTC62300.059.28689215
AGTCCTA74100.059.23295218
AAGGAGT156450.059.22170314
ATTCGAG57600.059.11292613
AAAGGAG217650.058.94842513
TTCGAGT49950.058.8360414
TGGAGTC171650.058.53639615
AGAGTCC89100.057.7314816
AGGAGTC136300.057.7190215
GAGTCCA106150.057.6981117
TAAACGA38550.057.01838712
AGTCCGA40000.056.77738618
Produced by FastQC (version 0.11.5)