FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6964950
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT549900.065.8956517
GGAGTCC476550.064.96113616
AGTCCTC386100.064.341618
CGAGTCC136850.063.76844416
TCGAGTC101700.063.68781715
ATTGGAG318300.062.37564513
TGGAGTC340350.062.18897215
ATTCGAG105650.061.86255613
GAGTCCA246200.061.7703617
TTCGAGT86050.061.61191614
AGAGTCC182200.061.3302116
TTAGTCC105750.061.08608216
GAGTCCG96050.060.8860817
GAGTACT122600.060.77446417
TTGGAGT284600.060.36894614
GTCCTCT204150.060.25609219
AGTCCTA141400.060.04621518
TTTAGTC118350.059.83940515
TAGAGTC107550.059.80338715
AGTACTC100000.058.89830818
Produced by FastQC (version 0.11.5)