FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10269097
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC300150.065.4367616
TCGAGTC224700.065.3358315
GAGTCCA494050.063.3593417
TTCGAGT178800.062.88206514
AGTCCTC631100.062.60146318
GAGTACT230700.062.3286217
GAGTCCT1015900.062.2961117
TAGAGTC200050.062.2151815
AGAGTCC327700.062.09909416
TTTAGTC199250.062.02888515
TTAGTCC182050.062.0288716
ATTCGAG196000.061.8194513
GGAGTCC873950.061.59473416
GAGTCCG161800.060.81503717
TAGTCCT209750.060.48417
TTGAGTC216500.059.78363815
AGTCCTA256550.059.66921218
AGTACTC162100.059.3979918
AGTCCAG417950.059.3229718
AGTCCGA109950.059.18145418
Produced by FastQC (version 0.11.5)