FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4542131
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG91630.20173350350309138No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA73250.16126791587472927No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA58860.1295867512407722No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC57840.1273411092722777No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG57200.12593207901753606No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA56210.12375248534223253No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC47700.1050167861737145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA1401.8189894E-1187.46597145
GAGTACC30850.060.9983561
TAACCGA6050.060.225579
ACCCATA36900.054.7657855
AGTACCC34000.054.757562
GTACCCA35600.052.2971233
CCCATAA35650.052.039076
GTACGTT9550.051.288944145
GTACCCG12200.049.964765
CTAGTCG6950.049.5724033
CATAATA40750.048.609068
TTCGTGT17600.046.303513
CGAGCCG7200.046.0056468
CTAGGAT16750.044.777355145
GCTTAGA25800.044.684886
TCGTGTT18600.044.1707734
ATAATAC42600.043.698899
GCTAGCC18050.043.6842351
TACCCAT42550.043.444114
CGTGTTC19000.043.2418145
Produced by FastQC (version 0.11.5)