FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005785110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005785110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872766
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG35420.18913201115355577No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA31360.16745284781974895No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA28860.1541036093137103No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG26510.14155532511803398No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC25270.13493410281903878No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA23390.12489547546249771No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC21660.11565780241631896No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG20990.11208020649670061No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC20430.10908997707134793No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA19340.10326970908271509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA304.50018E-9206.4042145
TAACCGA2250.065.020799
CGGCGAA1300.064.311435
GCTAGCC8650.060.4081341
TTCGTGT6600.060.1701623
CTAGGAT7450.059.84337145
ATTCGCG502.4477893E-455.7365766
ACCCATA13150.055.1007965
GCGCAAT1450.052.8536452
CTAGTCG3300.052.7808463
CGTGTTC7550.052.5990835
CGTATCG1704.5292836E-850.99398145
AAACGTA3800.048.885204145
GAGTACC12550.048.852771
GCAATCG1301.9463187E-1048.2335741
GCCGTTA1600.047.8986172
TCGTGTT8350.047.559654
AGTACCC13250.046.7976882
CGCGTGT2701.8189894E-1145.8676145
GTACCCA13800.044.9325643
Produced by FastQC (version 0.11.5)