FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29340455
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG200350.020.301142145
TATGCCG189350.018.264172145
TCGTATG235450.018.044542145
CGTATGC245750.017.465261145
CTCGTAT272150.017.236254145
CGCGTAT62900.015.906527145
GCGAAAT757150.015.732676145
ATCTCGT380400.015.609548145
ATCGCGT82700.015.42958145
CGAAATC701050.015.295586145
CGCGAAA823850.015.215764145
AATCGCG92600.015.189298145
AATCTCG448050.015.097398145
TCGCGTA73100.014.877188145
CCGCGAA937000.014.740164145
TCTCGTA323550.014.520465145
TCCGCGA1046350.014.190573145
GTATGCC288750.014.186439145
ATGCCGT202650.014.060245145
CTCCGCG1210650.013.642126145
Produced by FastQC (version 0.11.5)