FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22635098
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA507150.010.204111118-119
ATCGGAA470400.010.059782118-119
AGATCGG563100.09.728652118-119
ACACGTC173150.09.716432118-119
TAGATCG181700.09.619118118-119
TCGGAAG458450.09.569896118-119
CGGAAGA446300.09.304271118-119
CACGTCT186350.08.597501118-119
CGTCTGA136200.08.57686118-119
GCACACG251100.08.440257118-119
CACACGT217050.08.394861118-119
ACGTCTG164950.08.091087118-119
AAGATCG189350.07.9275484118-119
CAGATCG185550.07.657373118-119
AGAGCAC417650.07.508494118-119
AGCACAC369200.06.7065034118-119
GAGCACA418050.06.612529118-119
GAAGAGC561400.06.3430376118-119
GAGATCG229300.06.2093234118-119
AAGAGCA560700.05.9851665118-119
Produced by FastQC (version 0.11.5)