FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26304191
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA316950.012.393564118-119
GTCGTGT356100.011.957908118-119
GCGTCGT424600.011.92666118-119
AGCGTCG485500.011.729074118-119
CGTCGTG389050.011.6789055118-119
CGTGTAG292450.011.367745118-119
GAGCGTC561600.010.923361118-119
AGAGCGT625250.010.519997118-119
ATCGGAA1086900.09.991378118-119
AAGAGCG715150.09.983417118-119
TCGGAAG1028800.09.867718118-119
GATCGGA1179350.09.621656118-119
AGATCGG1299900.09.429359118-119
CGGAAGA969250.09.351114118-119
GTGTAGG342550.09.036722118-119
TAGATCG388350.09.004678118-119
TGTAGGG351750.08.259326118-119
CAGATCG385000.08.2565975118-119
AAGATCG411700.07.9450383118-119
GTAGGGA310300.07.93474118-119
Produced by FastQC (version 0.11.5)