FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45835515
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71500.025.36279548-49
TCGTATG72950.024.92921444-45
CTCGTAT73300.024.45848844-45
CGTATGC75600.023.9280746-47
TCTCGTA90250.019.58660142-43
ATGCCGT112350.016.74575248-49
ATCTCGT109400.016.3080642-43
AATCTCG125200.014.74102540-41
GCTAATC131600.013.50760738-39
GCCGTCT141800.013.36636850-51
TGCCGTC145650.012.96479550-51
CGTCTGA144500.012.87321916-17
GTATGCC151350.012.617908546-47
ATCGGAA304250.012.0758752
CCGTCTT154750.011.83036152-53
ACACGTC160300.011.78119912-13
TCGGAAG316700.011.7641323
GATCGGA317900.011.4897461
CGGAAGA333950.011.1704514
ACGTCTG179250.010.56098114-15
Produced by FastQC (version 0.11.5)