FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45835515
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA59200.025.81811346-47
GAGCGTC152550.023.5599299
GCCGTAT66900.023.30275246-47
AGAGCGT166000.021.5379168
CGTATCA73000.021.32625648-49
CCGTATC77350.020.21798348-49
TCGCCGT81600.018.87340444-45
AAGAGCG202500.018.0510757
TCGTGTA101600.017.53015914-15
GCGTCGT103600.017.29679510-11
CGTCGTG106900.016.820512-13
AGCGTCG111400.016.31761410-11
GTCGTGT114100.015.8619512-13
GATCGGA222100.015.6114081
CGTGTAG116250.015.58339714-15
GTCGCCG103400.015.57543144-45
ATCGGAA220800.015.5653052
TGGTCGC102650.015.09379542-43
TCGGAAG248650.014.3031483
CGGAAGA267900.013.3108724
Produced by FastQC (version 0.11.5)