FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56074914
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGT85550.023.3747881
ATTCGTG92200.021.4338022
GTCGTAT58250.017.02598674-75
TATTACG61050.016.22742378-79
TACGTAG63150.015.86254980-81
CGTATTA64350.015.59543376-77
GCGTAAT65800.014.87755692-93
CGTGGAT138700.014.4731285
AGTCGTA73250.013.89155472-73
GGCGTAA72950.013.53077990-91
ATTACGT79800.012.88640278-79
TTCGTGG154550.012.8684893
TCGTGGA162650.012.5150994
TTACGTA83050.012.31707380-81
CGTAATC82650.012.1013192-93
ACGTAGC90150.011.48139382-83
TCGTATT91200.011.36537974-75
GAGTCGT92400.011.13997572-73
TTGCACG95150.010.72399852-53
CGTAGCT100400.010.54460982-83
Produced by FastQC (version 0.11.5)