FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35154516
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363290.1033409192719365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG209850.014.579618145
GCTATAG640250.013.09034145
TAGGCTA945450.012.990251145
GTAGGCT1158150.012.563923145
CTATAGT566800.012.381927145
GCGATAG118600.012.287232145
AGGCTAT927450.012.2808485145
ATAGTGT456550.012.116548145
TATAGTG523450.012.022291145
GAGTGTA1940300.011.826262145
TAGGCGA193250.011.742688145
AGTGTAG1856900.011.740527145
TGTAGGC1516800.011.691516145
GGCTATA720750.011.457287145
CGATAGT95550.011.381598145
GGCGATA134300.010.472936145
AGAGTGT2285900.010.425219145
AAGAGTG2503250.010.252783145
AAAGAGT2691200.09.95431145
GTGTAGG5556000.09.892521145
Produced by FastQC (version 0.11.5)