FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005787225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30089885
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG141400.028.251595145
CGTATGC220000.018.98194145
TCGTATG208500.018.464146145
TATCTCG352600.018.032587145
TATGCCG165650.017.857042145
CGCGTAT58350.017.767895145
CTCGTAT239450.017.107021145
TCTCGTA289450.016.806984145
TCGCGTA69100.016.682451145
CTATCGC115500.015.253343145
AGCTATC718050.014.872666145
TATCGCG83000.014.674792145
ATCTCGT345200.014.575701145
GTATGCC265200.014.188439145
ATCGCGT80900.013.801076145
GAAGCTA952300.013.094696145
ATGCCGT188250.013.094373145
AAGCTAT858150.012.621999145
CTATCTC557250.012.33386145
TGCCGTC203050.011.818592145
Produced by FastQC (version 0.11.5)